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KMID : 0377619720230020169
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Korean Jungang Medical Journal
1972 Volume.23 No. 2 p.169 ~ p.175
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Studies on Enrichment Media of Vibrio Parahaemolyticus
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Abstract
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It is sometimes neccessary to enrich pathogenic organisms in feces and other specimens of patients suffering from enteric infections. It is also true in case of food poisoning due to Vibrio parahaemolyticus, especially in the later stage of the infection. As we experienced that already reported enrichment media for vibrios are not always satisfactory, some experiments were carried out to lead the development of better enrichment media.
V. parahaemolyticus can grow in the presence of sodium chloride in phosphate buffer and the growth was accelerated at around 3 per cent of sodium chloride. Even though this organism showed a luxariant growth in peptone water containing sodium chloride, Escherichia coli and other organisms also grew very well in peptone water. Therefore, the selective enrichment of V. parahaemolyticus over E. coli and other enteric flora would not be obtained in peptone water containing sodium chloride.
The growth of V. parahaemolyticus and total microorganisms in feces was compared in the presence of sodium chloride in phosphate buffer and in peptone water, and the results showed that the growth rate of V. parahaemolyticus was more prominent than that of total microorganisms in phosphate buffer containing 3-5 per cent sodium chloride than in peptone water containing sodium chloride. From these results, we propose the successful enrichment of V. parahaemolyticus in phosphate buffer containing 3-5 per cent sodium chloride.
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